Figure 4 | Scientific Reports

Figure 4

From: DNA methyltransferase inhibition induces dynamic gene expression changes in lung CD4+ T cells of neonatal mice with E. coli pneumonia

Figure 4

DAC treatment of neonatal mice infected with E. coli induces a juvenile-like immune response in CD4+ T cells. (A) Multi-level computational filtering overview. Genes were filtered to include only those that show a change in methylation state in response to DAC treatment in either age group. These differentially methylated genes were then ranked by their differential mRNA expression when comparing neonatal DMSO to all other groups. (B) Matrixplot of scaled mRNA expression levels for the top DEGs in each direction in the comparison of neonatal DMSO versus all else, generated with scanpy28. Bolded genes are directly involved in type-I interferon responses. Figure restricted to top 25 genes for visibility. (C) Over-Enrichment Analysis (ORA) of GO:BPs terms for the top DEGs identified in (B), showing over-representation of terms related to viral and bacterial defense responses. (D) ORA as in (C) for transcription factor (TF) motifs, showing over-representation of interferon response factor (IRF) motifs in the promoters of up-regulated DEGs.

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