Figure 1
Quinine induced off-response of PKD2L1 channel. (a) Whole-cell PKD2L1 current recorded at − 60 mV in HEK293T cells exogenously expressing PKD2L1. Quinine (100 µM) was added as indicated by an upper orange line. (b) Time-expanded traces from the horizontal bars (black, orange, and blue) in (a). The traces before (Control, black), during (Quinine, orange), and after (Wash, blue) application of quinine are shown. Dotted lines indicate closed (C) and open levels. The corresponding all-points amplitude histograms are shown on the right. PDF stands for probability density function. Gaussian fittings are shown in red lines. (c, d) The channel activity (NPo) (c) and the single-channel conductance (d) before (Control), during (Quinine), and after (Wash) application of quinine. The data are averaged from 20 experiments. P values are determined by one-way ANOVA with Tukey’s post-hoc analysis (F = 17.59 in c and F = 0.24 in d). *, P < 0.05. NS, P > 0.05. (e) Concentration dependence of quinine on the off-response of the PKD2L1 channel activities at − 60 mV. The data are averaged from 6 to 18 experiments.
