Figure 1 | Scientific Reports

Figure 1

From: Unchanged PCNA and DNMT1 dynamics during replication in DNA ligase I-deficient cells but abnormal chromatin levels of non-replicative histone H1

Figure 1

LigI is not essential for PCNA deposition on and unloading from replicating DNA. DNA ligase I and GAPDH detected by immunoblotting in extracts (50 μg) from; (a) human LigI deficient fibroblast cell line 46BR.1G1 (VC) and a derivative stably expressing wild type LigI (WTC) and; (c) mouse B cell line CH12F3 (WT) and a LIG1 null derivative (Δ/Δ). The human (b) and mouse (d) cell lines were processed by aniPOND and immunoblotted for PCNA. CAP = 50% of beads capture proteins. INP = 1% of pre-pulldown proteins. Samples indicated as T0, T30, and T60 correspond to a 15 min EdU pulse followed by 0, 30, and 60 min thymidine chase, respectively. Uncropped Western blots are shown in supplementary Fig S6.

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