Figure 2 | Scientific Reports

Figure 2

From: Unchanged PCNA and DNMT1 dynamics during replication in DNA ligase I-deficient cells but abnormal chromatin levels of non-replicative histone H1

Figure 2

The absence of LigI does not alter the kinetics of PCNA, the PCNA loader (RFC1) and the PCNA unloader (ATAD5) association with newly synthesized DNA. CH12F3 wild type cells and a LIG1 null derivative grown in media containing light (WT:L, blue line) or heavy (LigI:H, red line) isotope labeled amino acids, respectively, were pulse labeled with EdU for 15 min followed by incubation with thymidine containing media for 0 (T0), 30 (T30) and 60 (T60) min. After processing by aniPOND, the relative levels of; (a) LigI; (b) PCNA; (c) RFC1; (d) ATAD5; (e) RFC2; (f) RFC3; (g) RFC4; and (h) RFC5 associated with EdU-labeled DNA were determined by mass spectrometry as described in Materials and Methods.

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