Figure 7 | Scientific Reports

Figure 7

From: Unchanged PCNA and DNMT1 dynamics during replication in DNA ligase I-deficient cells but abnormal chromatin levels of non-replicative histone H1

Figure 7

Increased levels of chromatin bound H1.0 in Ligase I deficient cells. (a) Immunoblots of whole cell extracts (50 μg, left panel) and chromatin fractions (50 μg, middle panel) from CH12F3 wild type cells (WT) and a LIG1 null derivative (Δ/Δ) as well as the LIG1 null cells expressing either Flag-tagged wild type LigI (KO WT) or the TARK mutant version (KO K126R) with the indicated antibodies. (b) Immunoblots of whole cell extracts (50 μg, left panel) and chromatin fractions (50 μg, middle panel) from LigI-deficient 46BR.1G1 cells (46BR.1G1), a complemented derivative of 46BR.1G1 cells stably expressing wild type LigI (7A3) and a comparable wild type human fibroblast (MRC-5V1). Data shown graphically are the mean ( ±) SEM of three independent experiments. Unpaired two-tailed Student test. ‘*’ P-values < 0.05. Uncropped Western blots are shown in supplementary Fig S9.

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