Table 2 COI haplotype frequencies for Rana temporaria populations in the Province of Trento detected by eDNA metabarcoding across temporal replicates. For each wetland site, haplotype frequencies (mean of spatial replicates S1-3) detected by eDNA metabarcoding at the different temporal points (T1, T2 and mean T1 + T2) are reported, together with available frequencies derived from traditional tissue-based genetic sampling (reference dataset: 41). nd = no data.
Site | Dataset | COI haplotypes (frequencies) | |||||||
---|---|---|---|---|---|---|---|---|---|
CA2_TN4 | VC6 | PR4 | SA1_PR7 | TN2 | TN3 | TN5 | MT5 | ||
Amp | eDNA (T1) | 0.644 | 0 | 0 | 0 | 0.74 | 0 | 0 | 0 |
eDNA (T2) | 0.317 | 0 | 0 | 0 | 0.68 | 0 | 0 | 0 | |
eDNA (T1 + T2) | 0.2907 | 0 | 0 | 0 | 0.71 | 0 | 0 | 0 | |
reference dataset | 0.4 | 0 | 0 | 0 | 0.6 | 0 | 0 | 0 | |
Bed | eDNA (T1) | 0.2637 | 0.61 | 0.122 | 0 | 0 | 0 | 0 | 0 |
eDNA (T2) | 0.2006 | 0.59 | 0.209 | 0 | 0 | 0 | 0 | 0 | |
eDNA (T1 + T2) | 0.2321 | 0.6 | 0.166 | 0 | 0 | 0 | 0 | 0 | |
reference dataset | 0.1 | 0.8 | 0.1 | 0 | 0 | 0 | 0 | 0 | |
Fia | eDNA (T1) | 0.3871 | 0 | 0 | 0 | 0.16 | 0.43 | 0.0196 | 0 |
eDNA (T2) | 0.4415 | 0 | 0 | 0 | 0.06 | 0.49 | 0 | 0 | |
eDNA (T1 + T2) | 0.4089 | 0 | 0 | 0 | 0.12 | 0.46 | 0.0118 | 0 | |
reference dataset | 0.3 | 0 | 0 | 0 | 0.1 | 0.3 | 0.3 | 0 | |
Ing | eDNA (T1) | 0.9528 | 0.05 | 0 | 0 | 0 | 0 | 0 | 0 |
eDNA (T2) | 0.2345 | 0.77 | 0 | 0 | 0 | 0 | 0 | 0 | |
eDNA (T1 + T2) | 0.7133 | 0.29 | 0 | 0 | 0 | 0 | 0 | 0 | |
reference dataset | 0.8 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | |
Lel | eDNA (T1) | 0.2203 | 0.78 | 0 | 0 | 0 | 0 | 0 | 0 |
eDNA (T2) | nd | nd | nd | nd | nd | nd | nd | nd | |
eDNA (T1 + T2) | 0.2203 | 0.78 | 0 | 0 | 0 | 0 | 0 | 0 | |
reference dataset | 0.5 | 0.4 | 0 | 0.1a | 0 | 0 | 0 | 0 | |
PLa | eDNA (T1) | 0.8031 | 0 | 0.134b | 0.0628 | 0 | 0 | 0 | 0 |
eDNA (T2) | nd | nd | nd | nd | nd | nd | nd | nd | |
eDNA (T1 + T2) | 0.8031 | 0 | 0.134b | 0.0628 | 0 | 0 | 0 | 0 | |
reference dataset | 0.8 | 0 | 0 | 0.2 | 0 | 0 | 0 | 0 | |
PMa | eDNA (T1) | 0.1754 | 0.82 | 0 | 0 | 0 | 0 | 0 | 0 |
eDNA (T2) | nd | nd | nd | nd | nd | nd | nd | nd | |
eDNA (T1 + T2) | 0.1754 | 0.82 | 0 | 0 | 0 | 0 | 0 | 0 | |
reference dataset | 0.7 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | |
PS1 | eDNA (T1) | 0.3936 | 0.35 | 0.26 | 0 | 0 | 0 | 0 | 0 |
eDNA (T2) | 0.7635 | 0 | 0.237 | 0 | 0 | 0 | 0 | 0 | |
eDNA (T1 + T2) | 0.5169 | 0.23 | 0.252 | 0 | 0 | 0 | 0 | 0 | |
reference dataset | 0.3 | 0.2 | 0.5 | 0 | 0 | 0 | 0 | 0 | |
PS2 | eDNA (T1) | 0.6715 | 0.09 | 0.238 | 0 | 0 | 0 | 0 | 0 |
eDNA (T2) | nd | nd | nd | nd | nd | nd | nd | nd | |
eDNA (T1 + T2) | 0.6715 | 0.09 | 0.238 | 0 | 0 | 0 | 0 | 0 | |
reference dataset | 0.5 | 0.1 | 0.4 | 0 | 0 | 0 | 0 | 0 | |
Va1 | eDNA (T1) | 0.5235 | 0 | 0 | 0 | 0.24 | 0.19 | 0.0373b | 0 |
eDNA (T2) | 0.4887 | 0 | 0 | 0 | 0.1 | 0.25 | 0.1544b | 0 | |
eDNA (T1 + T2) | 0.5061 | 0 | 0 | 0 | 0.17 | 0.22 | 0.0959b | 0 | |
reference dataset | 0.4 | 0 | 0 | 0 | 0.1 | 0.2 | 0 | 0.3a |