Figure 3
From: Extracellular vesicles are dynamic regulators of maternal glucose homeostasis during pregnancy
Dynamic increase of circulating EV concentration shifts glucose dynamics. (A) Glucose tolerance curves for non-pregnant dams that received no injection (n = 7, black), vehicle injection (n = 5, green), or EV injection (n = 6, red) (two-way ANOVA, main effect of time, F2.61,39.14 = 122.7, p < 0.0001, main effect of injection, F2,15 = 1.265, p = 0.3106, time*injection interaction, F8,60 = 2.666, p = 0.0142). There was no significant difference in the glucose tolerance test (B) area under the curve (one-way ANOVA, main effect of injection, F2,15 = 2.02, p = 0.1672, Bartlett’s test, p = 0.2105, SDNoInj = 2296, SDVehInj = 2162, SDEVInj = 1005), (C) change in glucose from 0 to 15 min (one-way ANOVA, main effect of injection, F2,15 = 2.474, p = 0.1179, Bartlett’s test, p = 0.5110, SDNoInj = 44.05, SDVehInj = 29.23, SDEVInj = 27.52), or (D) slope of glucose processing from 30 to 60 min (one-way ANOVA, main effect of injection, F2,15 = 2.922, p = 0.0848, Bartlett’s test, p = 0.4621, SDNoInj = 1.045, SDVehInj = 0.7723, SDEVInj = 0.5935). (E–G) Individual dam glucose tolerance curves are shown (E) for non-injected dams, (F) vehicle injected dams, and (G) EV injected dams.
