Table 1 Analytical performance of 93 optimized assay-sets in the high-throughput BioMark HD nano-fluidic real-time PCR (‘Fluidigm’).
Assay-set | Calibrator | Efficiency (%) [− 1 + 10(−1/m)] | LOD (g-Block) | Linear equation | r2 |
|---|---|---|---|---|---|
1 | St 1 | 98 | 101 | y = − 3.3761x + 24.236 | 1.00 |
2 | St 2 | 95 | 10 | y = − 3.4361x + 26.784 | 1.00 |
3 | St 3 | 108 | 101 | y = − 3.1449x + 22.45 | 1.00 |
4 | St 4 | 100 | 101 | y = − 3.32x + 28.393 | 1.00 |
5 | St 5 | 99 | 101 | y = − 3.3421x + 25.415 | 1.00 |
6A/B/C/D(F/G/H) | St 6B | 100 | 101 | y = − 3.327x + 30.357 | 0.99 |
6A/C | St 6C | 97 | 102 | y = − 3.4039x + 30.277 | 0.97 |
6C/D | St 6C | 97 | 10 | y = − 3.384x + 27.769 | 0.99 |
7A/F | St 7F | 95 | 101 | y = − 3.4605x + 26.292 | 1.00 |
7B/C/40 | St 7C | 102 | 101 | y = − 3.2729x + 27.595 | 0.99 |
8 | St 8 | 110 | 102 | y = − 3.1071x + 24.264 | 1.00 |
9A/L/N/V | St 9A | 102 | 101 | y = − 3.268x + 25.114 | 1.00 |
9A/V | St 9 V | 101 | 101 | y = − 3.3014x + 24.495 | 0.99 |
9L/N | St 9N | 99 | 10 | y = − 3.3583x + 28.397 | 1.00 |
10A | St 10A | 99 | 10 | y = − 3.3555x + 24.534 | 1.00 |
10A/B | St 10B | 100 | 101 | y = − 3.3263x + 23.409 | 0.99 |
10C/F | St 10C | 96 | 102 | y = − 3.4327x + 28.19 | 1.00 |
11A/B/C/D/E/F | St 11A | 102 | 101 | y = − 3.2846x + 26.706 | 0.99 |
11A/D | St 11D | 91 | 102 | y = − 3.5656x + 29.722 | 1.00 |
11B/C | St 11B | 100 | 102 | y = − 3.3137x + 25.745 | 1.00 |
11F | St 11F | 103 | 102 | y = − 3.2412x + 30.921 | 0.99 |
12A/B/F/44/46 | St 12B | 100 | 102 | y = − 3.3217x + 22.458 | 1.00 |
12B | g-Block | 97 | 101 | y = − 3.4084x + 26.027 | 1.00 |
13 | St 13 | 105 | 10 | y = − 3.2135x + 24.699 | 1.00 |
14 | St 14 | 100 | 102 | y = − 3.3232x + 26.189 | 0.99 |
15A/B/C/F | St 15A | 97 | 10 | y = − 3.3908x + 26.255 | 1.00 |
15A/F | St 15A | 109 | 102 | y = − 3.119x + 23.396 | 0.99 |
15B/C | St 15B | 98 | 102 | y = − 3.3707x + 25.31 | 0.99 |
16A | g-Block | 102 | 10 | y = − 3.2674x + 24.3 | 0.99 |
16F | St 16F | 104 | 102 | y = − 3.2321x + 25.116 | 1.00 |
16F/18F/28AF | St 18F | 108 | 101 | y = − 3.1525x + 24.213 | 0.99 |
17A | St 17A | 91 | 101 | y = − 3.5508x + 23.693 | 1.00 |
17A/F | St 17F | 99 | 101 | y = − 3.3389x + 26.05 | 0.98 |
18A/B/C/F | St 18C | 99 | 102 | y = − 3.3484x + 22.148 | 1.00 |
18B/C/F | St 18C | 98 | 10 | y = − 3.363x + 23.995 | 1.00 |
18C/F | St 18C | 98 | 102 | y = − 3.3743x + 31.732 | 0.99 |
19A | St 19A | 94 | 102 | y = − 3.483x + 23.801 | 1.00 |
19B/F | St 19B | 108 | 102 | y = − 3.1345x + 20.812 | 1.00 |
19F | g-Block | 110 | 101 | y = − 3.1075x + 26.863 | 1.00 |
20 | St 20 | 99 | 102 | y = − 3.3405x + 25.595 | 0.99 |
21 | St 21 | 103 | 102 | y = − 3.2409x + 27.273 | 0.99 |
22A/F | St 22F | 98 | 102 | y = − 3.3701x + 26.527 | 1.00 |
22F | St 22F | 95 | 102 | y = − 3.438x + 30.669 | 0.98 |
23A/B/F | St 23B | 94 | 102 | y = − 3.4648x + 29.288 | 0.98 |
23B | St 23B | 97 | 102 | y = − 3.3888x + 28.063 | 1.00 |
23F | St 23F | 102 | 101 | y = − 3.2754x + 22.908 | 0.99 |
24A | St 24A | 94 | 101 | y = − 3.4846x + 25.594 | 0.99 |
24B/F | St 24F | 103 | 102 | y = − 3.2571x + 25.548 | 1.00 |
25A/F | St 25F | 90 | 101 | y = − 3.5977x + 23.92 | 0.99 |
25A/F/38 | St 25A | 93 | 102 | y = − 3.5069x + 24.259 | 1.00 |
27 | St 27 | 95 | 102 | y = − 3.4509x + 24.242 | 0.99 |
28AF | g-Block | 92 | 102 | y = − 3.517x + 24.565 | 1.00 |
29 | g-Block | 105 | 102 | y = − 3.2145x + 26.526 | 1.00 |
31 | g-Block | 104 | 10 | y = − 3.2831x + 22.833 | 1.00 |
32A/F | St 32A | 98 | 10 | y = − 3.3804x + 22.641 | 0.99 |
33A/F/37 | St 33A | 100 | 10 | y = − 3.3257x + 23.074 | 0.99 |
33B/C | St 33C | 97 | 10 | y = − 3.406x + 25.401 | 1.00 |
33C | St 33C | 96 | 101 | y = − 3.4155x + 25.436 | 1.00 |
33D | g-Block | 109 | 10 | y = − 3.1097x + 25.098 | 1.00 |
34/17A | St 34 | 97 | 10 | y = − 3.4042x + 24.341 | 1.00 |
34/37/17A | St 37 | 100 | 10 | y = − 3.3282x + 27.087 | 1.00 |
35A/C/42 | St 35C | 100 | 10 | y = − 3.3161x + 25.966 | 0.99 |
35B | St 35B | 103 | 101 | y = − 3.251x + 27.268 | 0.98 |
35F/47F | St 35F | 90 | 102 | y = − 3.5757x + 24.976 | 0.98 |
36 | St 36 | 102 | 101 | y = − 3.2814x + 26.331 | 1.00 |
39 | g-Block | 106 | 10 | y = − 3.462x + 21.292 | 1.00 |
41A | St 41A | 101 | 10 | y = − 3.3039x + 24.147 | 1.00 |
41F | g-Block | 94 | 102 | y = − 3.4843x + 22.181 | 0.98 |
43 | g-Block | 105 | 10 | y = − 3.1997x + 27.245 | 0.99 |
45 | St 45 | 90 | 10 | y = − 3.6009x + 26.052 | 1.00 |
46 | g-Block | 98 | 101 | y = − 3.3655x + 21.273 | 1.00 |
47AF | St 47F | 95 | 101 | y = − 3.442x + 24.074 | 0.99 |
48 | St 48 | 91 | 10 | y = − 3.5707x + 24.586 | 0.99 |
Acinetobacter baumanii | A. baumanii | 94 | 101 | y = − 3.4785x + 26.193 | 0.99 |
Bordetella holmesii (hIS) | g-Block | 91 | 102 | y = − 3.5491x + 29.271 | 1.00 |
B. pertussis/holmesii (IS481) | B. pertussis | 90 | 102 | y = − 3.5933x + 25.235 | 0.99 |
B. parapertussis (pIS) | B. parapertussis | 95 | 10 | y = − 3.4358x + 21.956 | 0.99 |
B. pertussis/bronchiseptica/parapertussis (PtxS) | B. parapertussis | 97 | 10 | y = − 3.3903x + 22.329 | 0.99 |
Haemophilus influenzae (BexA) | H. influenzae, type b | 93 | 101 | y = − 3.513x + 25.169 | 1.00 |
H. influenzae (BexB) | H. influenzae, type b | 102 | 101 | y = − 3.2784x + 26.106 | 1.00 |
H. influenzae, type b | H. influenzae, type b | 97 | 102 | y = − 3.3953x + 22.967 | 0.99 |
H. influenzae (IgA1) | H. influenzae, type b | 97 | 10 | y = − 3.3905x + 21.628 | 1.00 |
Klebsiella pneumoniae | g-Block | 99 | 10 | y = − 3.3499x + 26.09 | 0.98 |
Morexella catarrhalis | M. catarrhalis | 97 | 101 | y = − 3.4079x + 26.561 | 0.99 |
Neiserria lactamica | N. lactamica | 102 | 101 | y = − 3.2662x + 25.872 | 0.98 |
Neiserria meningitidis | N. meningitidis | 99 | 102 | y = − 3.3496x + 24.255 | 0.98 |
Staphylooccus aureus | S. aureus | 91 | 101 | y = − 3.5547x + 29.696 | 1.00 |
Streptococcus oralis | g-Block | 108 | 101 | y = − 3.1503x + 26.386 | 1.00 |
Streptococcus pneumoniae (LytA) | St 4 | 99 | 101 | y = − 3.3582x + 26.927 | 1.00 |
S. pneumoniae (PiaB) | St 4 | 97 | 101 | y = − 3.394x + 26.863 | 0.99 |
S. pneumoniae (Ply) | St 4 | 96 | 101 | y = − 3.4107x + 26.927 | 1.00 |
S. pyogenes | g-Block | 99 | 10 | y = − 3.3488x + 25.05 | 1.00 |
