Figure 2 | Scientific Reports

Figure 2

From: Synchronized proinsulin trafficking reveals delayed Golgi export accompanies β-cell secretory dysfunction in rodent models of hyperglycemia

Figure 2

Co-localization marker analysis of proCpepRUSH trafficking. INS1 832/3 cells expressing AdRIP-proCpepRUSH were treated with biotin (200 μM) to initiate proCpepRUSH (green) trafficking for the indicated times prior to fixation. Cells were immunostained for GRP94 (red) (A), CPE (red) (B), or mannose-6-phosphate receptor (M6PR, red) (C). (A-C) TGN38 (magenta) and DAPI (blue) were used as counterstains. Boxed insets are shown with additional magnification. Scale bar = 5 μm.

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