Figure 4
Gross and microscopic lesions and in situ hybridization (ISH) using PCV4-specific probe targeting Cap gene of PCV4. Grossly, lung failed to collapse with dark red to purple consolidation and diffuse fibrinous attachment in the pleura (A). Lung: PCV4-positive cells were characterized by pink to brilliant red in the cytoplasm of bronchial epithelial cells (arrows) (B). Tracheobronchial lymph node: positive signals were observed in lymphocytes and histiocyte-like cells in the lymphoid follicle (arrows) (C). No hybridization signals were seen in the internal negative control of lung and tracheobronchial lymph node (D,E).
