Figure 2 | Scientific Reports

Figure 2

From: Small RNA sequencing of circulating small extracellular vesicles microRNAs in patients with amyotrophic lateral sclerosis

Figure 2

Differential expression of sEV miRNAs and the results of droplet digital PCR validation. Venn diagram (A) and heatmaps (B) showing the number and differential expression of miRNAs identified in a discovery set by each method (DESeq2, edgeR, and quantile normalization with independent t test). Expression values were normalized across all samples (columns: P, ALS patients; C, healthy controls) by Z-score, and miRNA clustering (row) was applied. (C) Quantification of levels of five miRNAs (commonly identified in the three analyses) by droplet digital PCR in a validation set. The amplified droplets were analyzed using Quantasoft software v1.7.4 (https://www.bio-rad.com/webroot/web/pdf/lsr/literature/10047467.pdf). Each bar indicates the relative mean quantity of each miRNA (normalized to miR-191-5p as an endogenous reference) compared to the average value in the healthy control. Error bars and asterisk (*) represent standard error of the mean and statistically significant difference (p < 0.05, Wilcoxon rank sum test).

Back to article page