Figure 6 | Scientific Reports

Figure 6

From: Heat-inactivated Factor B inhibits alternative pathway fluid-phase activation and convertase formation on endothelial cell-secreted ultra-large von Willebrand factor strings

Figure 6

Quantification of GMVEC-released complement proteins attached to GMVEC-secreted ULVWF strings in the presence or absence of HFB. Intensities of GMVEC-released complement proteins were measured along ULVWF strings secreted in response to: (a) 100 µM histamine; and (b) histamine ± 20 µg/ml HFB. Shown are the complement component fluorescent intensities per micron of ULVWF string length after background string subtraction. Values are means plus SD; n = 65–151 strings for proteins without HFB (blue bars) and 151–320 strings for proteins with HFB (red bars). Data were compiled from 3 to 9 experiments per condition and over 800 fluorescent images. In (a), values for C3, FB and C5 are statically different from C4 (****P < 0.0001); and in (b), paired comparison values for C3 and C3 + HFB (****P < 0.0001), C5 and C5 + HFB (****P < 0.0001) and FB and FB + HFB (*P = 0.0311) are statistically different by one-way ANOVA, non-parametric analysis.

Back to article page