Table 1 Concentrations of AP proteins and HFB in reactions using purified complement components.

From: Heat-inactivated Factor B inhibits alternative pathway fluid-phase activation and convertase formation on endothelial cell-secreted ultra-large von Willebrand factor strings

Reaction description

Reaction concentration, ng/µl

HFB to FB ratio

FB

HFB

C3

FD

1. 10.4 ng/µl FB, C3, FD

10.4

none

60

0.07

 

2. 10.4 ng/µl HFB, C3, FD

none

10.4

60

0.07

 

3. 10.4 ng/µl HFB, FB, C3, FD

10.4

10.4

60

0.07

1-fold

4. 15.6 ng/µl HFB, FB, C3, FD

10.4

15.6

60

0.07

1.5-fold higher

5. 20.8 ng/µl HFB, FB, C3, FD

10.4

20.8

60

0.07

2-fold higher

6. 26 ng/µl HFB, FB, C3, FD

10.4

26

60

0.07

2.5-fold higher

7. 31.2 ng/µl HFB, FB, C3, FD

10.4

31.2

60

0.07

3-fold higher

8. No FD, HFB, FB, C3

10.4

10.4

60

none

1-fold

  1. The description summarizes the proteins included in the reaction and the changes in HFB concentration. Reaction concentrations for each protein are in the center columns. The HFB to FB ratio describes changes in HFB concentrations to FB concentration. The reactions were in EGTA/MgCl2 buffer and activation occurred over 15 min at 37 °C. The results are shown in Fig. 1.
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