Table 2 FH additions and concentrations in FH-D serum reactions.

From: Heat-inactivated Factor B inhibits alternative pathway fluid-phase activation and convertase formation on endothelial cell-secreted ultra-large von Willebrand factor strings

Reaction description

FH levels in reactions

FH, ng/µl

% Normal serum levels

1. FH-D serum alone

0

 

2. FH-D serum + 0.78 ng/µl FH

0.78

3

3. FH-D serum + 1.56 ng/µl FH

1.56

6

4. FH-D serum + 3.13 ng/µl FH

3.13

12.5

5. FH-D serum + 6.25 ng/µl FH

6.25

25

6. FH-D serum + 12.5 ng/µl FH

12.5

50

7. FH-D serum + 25 ng/µl FH

25

100

  1. AP activation was measured in FH-depleted serum reactions with the addition of increasing amounts of FH. Table 3 shows the final FH concentrations in the FH-D serum reactions, along with comparable FH levels in normal serum. C3a and Ba levels were measured after 15 min at 37 °C. The results are in Fig. 2.
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