Table 3 FH and HFB concentrations in FH-D serum reactions.

From: Heat-inactivated Factor B inhibits alternative pathway fluid-phase activation and convertase formation on endothelial cell-secreted ultra-large von Willebrand factor strings

Reaction description

Reaction conc., ng/µl

HFB to FB ratio

FH

HFB

1. FH-D serum alone

0

0

–

2. FH-D serum, 3.13 FH

3.13

0

–

3. FH-D serum, 3.13 FH, 20.8 HFB

3.13

20.8

2-fold

4. FH-D serum, 3.13 FH, 26 HFB

3.13

26

2.5-fold

5. FH-D serum, 3.13 FH, 31.2 HFB

3.13

31.2

3-fold

6. FH-D serum, 3.13 FH, 36.4 HFB

3.13

36.4

3.5-fold

7. FH-D serum, 36.4 HFB

0

36.4

3.5-fold

8. FH-D serum, without Mg+2 ions

0

0

–

  1. Protein concentrations of FH and HFB in reactions containing FH-D serum, low FH and increasing amounts of HFB. In reactions, the FH-D serum was diluted in 0.1 mM EDTA to produce final concentrations of 60 ng/µl C3 and 10.4 ng/µl FB. The FH concentration was equivalent to 12.5% of normal serum levels. C3a and Ba levels were measured after 5 min at 22 °C. The results are in Fig. 3. The values in the Reaction description are protein concentrations in ng/µl.
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