Figure 1

Rumen pH (A) and upstream denoising pipeline output metrics displaying total sequences (B) number of chimeras (C) unused sequences (D) amongst oro-esophageal tubing and the respective fractions from rumen cannula. Statistical differences across group means were declared at P ≤ 0.05. Different superscripts mean groups differ through the Tukey–Kramer test performed at P ≤ 0.05 significance level.