Figure 5
Partial least square-discriminant analysis (PLS-DA) of ruminal metabolites from ruminal samples collected through an oro-esophageal tubing and the respective fractions from the rumen cannula. A 2-D representation of differences in known (A) and unknown (B) metabolome composition is displayed for the illustration of how closely related the oro-esophageal tubing metabolome samples are to the respective fractions from the rumen cannula. A 3-D representation of known (C) and unknown (D) metabolome composition is displayed to demonstrate that there are still differences between the oro-esophageal tubing procedure and rumen cannula metabolome samples that need to be considered in future studies.
