Figure 6

Hierarchical clustering heatmap showing top 50 metabolites detected through analysis of variance to differ in rumen sampling methods. Color differences indicate the Pearson correlation of metabolite ion abundances and sampling method. Wards clustering method was used to assess similarity among sampling methods and is displayed at the top portion of the heatmap. Statistical differences were declared at P ≤ 0.05. Heatmap was produced on Metaboanalyst 5.0 (https://www.metaboanalyst.ca/).