Figure 7

Enrichment pathway analysis was performed in Metaboanalyst 5.0 using the KEGG pathway database45,46,47. The figure displays the top 25 most enriched pathways (A) that differed between the oro-esophageal tubing procedure and a complete sample from the rumen cannula (fluid and particulate together). (B) graphical visualization of metabolite differences between the two sampling techniques within some of the top 25 most enriched pathways. Statistical differences were declared at P ≤ 0.05.