Table 4 Application of the standard addition technique to examine the matrix effect.
HP-TLC method | RP-HPLC method | ||||||||
|---|---|---|---|---|---|---|---|---|---|
Taken (µg/band) | Founda% | Pure added (µg/band) | Pure found (µg/band) | Recovery % | Taken (µg/mL) | Founda% | Pure added (µg/mL) | Pure found (µg/band) | Recovery % |
HTX | |||||||||
0.10 | 100.09 | 0.05 | 0.05 | 99.76 | 1.00 | 100.13 | 0.50 | 0.50 | 100.98 |
0.10 | 0.10 | 98.95 | 1.00 | 1.00 | 99.59 | ||||
0.15 | 0.15 | 100.56 | 2.00 | 1.98 | 99.00 | ||||
Mean ± SD | 99.76 ± 0.81 | Mean ± SD | 99.85 ± 1.02 | ||||||
IDD | |||||||||
0.80 | 99.52 | 0.50 | 0.50 | 99.57 100.20 98.75 | 8.00 | 99.61 | 5.00 | 4.94 | 98.85 |
0.80 | 0.80 | 8.00 | 8.02 | 98.40 | |||||
1.00 | 0.99 | 10.00 | 9.84 | 100.28 | |||||
Mean ± SD | 99.51 ± 0.72 | Mean ± SD | 99.18 ± 0.98 | ||||||
TTM | |||||||||
0.40 | 99.91 | 0.20 | 0.20 | 100.77 | 3.00 | 99.59 | 2.00 | 1.99 | 99.34 |
0.40 | 0.40 | 100.54 | 3.00 | 3.02 | 100.51 | ||||
0.60 | 0.60 | 99.25 | 5.00 | 4.94 | 98.86 | ||||
Mean ± SD | 100.19 ± 0.82 | Mean ± SD | 99.57 ± 0.85 | ||||||
