Figure 1 | Scientific Reports

Figure 1

From: Functional and phosphoproteomic analysis of β-adrenergic receptor signaling at excitatory synapses in the CA1 region of the ventral hippocampus

Figure 1

LTP induction in the ventral hippocampal CA1 region requires β-adrenergic receptor activation. (A) A 30-s-long train of TPS (delivered at time = 0) induced robust LTP in dorsal hippocampal slices (fEPSPs were potentiated to 150 ± 5% of baseline, n = 12) but had no lasting effect on synaptic strength in ventral hippocampal slices (fEPSPs were 105 ± 3% of baseline, n = 11). Traces show superimposed fEPSPs recorded during baseline and 45 min post-TPS in dorsal and ventral slices. (B) Scatter plot shows fEPSP slopes 45 min post-TPS in all experiments (t(21) = 7.447, **p = 2.55 × 10–7). (C) Synaptic transmission in ventral hippocampal slices was not persistently altered by either TPS alone (30 s duration, top) or bath application of 1.0 µM ISO alone (bottom). Field EPSPs were 101 ± 2% of baseline 45 min after TPS (n = 5) and 101 ± 3% of baseline 45 min after ISO application (n = 5). (D) TPS delivered at the end of a 10-min bath application of ISO (indicated by the bar) induced robust LTP (fEPSPs were potentiated to 176 ± 12% of baseline, n = 16). Traces show superimposed fEPSPs recorded during baseline and 45 min post-TPS alone, ISO alone, or TPS delivered in the presence of ISO. (E) Scatter plot shows fEPSP slopes 45 min post-TPS or ISO application in all experiments. **p < 0.005, one-way ANOVA with post hoc Bonferroni t-tests, F(2,23) = 11.396, p < 0.001.

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