Figure 7

miR-147a negatively regulated expression levels of MEF2A and VEGFA via direct a binding relationship. (A) Starbase was employed to predict the complementary binding site between miR-147a and MEF2A. (B) Dual-luciferase reporter gene assay was carried out to analyze the interaction between miR-147a and MEF2A. (C) miR-147a and MEF2A expressions in HaCaT cells following miR-147a mimics/inhibitor transfection were assessed using RT-qPCR. (D) The protein level of MEF2A in HaCaT cells following miR-147a mimics/inhibitor transfection was assessed using western blot. (E) The expression correlation of miR-147a and MEF2A in the serum and skin tissues of AD mice was analyzed by Spearman correlation analysis. (F) Starbase was employed to predict the complementary binding site between miR-147a and VEGFA. (G) Dual-luciferase reporter gene assay was carried out to analyze the interaction between miR-147a and VEGFA. (H) miR-147a and VEGFA expressions in HUVECs following miR-147a mimics/inhibitor transfection were assessed using RT-qPCR. (I) VEGFA protein level in HUVECs following miR-147a mimics/inhibitor transfection was assessed using western blot. Data were expressed as mean ± SD. All our data were obtained from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.