Figure 2
Tarsal joint measurements and inflammation are similar among treatment groups, while serum IL2 levels vary significantly. Mice were infected with CHIKV subcutaneously in the left hind foot near the tarsal joint. PBS, rIL2, anti-IL2 mAB or complex were administered to mice on days 16–18 post-CHIKV infection. Error bars represent SEM. (a) Average joint size of mice by treatment group on each day of the study, starting one day before infection (day -1), which served as the baseline joint size for calculating inflammation. The average joint size was calculated by multiplying the height by the breadth of the left tarsal joint. (b) Tarsal joint inflammation was calculated using [(x – day -1)/day -1)], where x is the footpad size measurement for a given day post-infection and day -1 is the day before infection. (c) Serum IL2 concentrations were measured on days 2, 14, and 19 after infection and were normalized using log10(IL2 + 1). The shaded region denotes the treatment period.
