Figure 3
Therapeutic complex treatment significantly increases the frequency and activation of regulatory T cells. Mice were infected with CHIKV and treated with IL2-based treatments on days 16–18 post-infection, and splenocytes were collected and stained for flow cytometry on day 19 post-infection. (a) Representative scatterplots show CD4 and FoxP3 gated on CD4 + CD25 + live splenocytes. The box denotes the FoxP3 + populations. (b) Representative scatterplots show CD25 and FR4 gated on CD4 + CD25 + FR4 + cells. Q1 is the CD25hiFR4lo activated Teff population, Q2 is the CD25hiFR4hi activated Treg population, and Q4 is the CD25loFR4lo naïve T cell population. (c) CD4 + CD25hiFoxP3 + Treg cell frequency out of live splenocytes. (d) FR4hiCD25hi activated Treg cell frequency out of live splenocytes. (e) Frequency of Helios-positive Tregs out of all Tregs. (f) CD25 median fluorescence intensity (MFI) on Tregs. (g) Helios MFI on Helios + Tregs. *Significant change from control (*p < 0.05, **p < 0.001, ***p < 0.0001).
