Figure 4
Anti-IL2 mAB and complex treatments significantly increase the frequency and activity of CD4 + effector T cells. Mice were infected with CHIKV and treated with IL2-based treatments on days 16–18 post-infection, and splenocytes were collected and stained for flow cytometry on day 19 post-infection. (a) Representative scatterplots show CD4 and CD25 gated on FoxP3- live splenocytes. The box denotes the CD4 + CD25 + FoxP3- populations. (b) Frequency of CD4 + CD25 + FoxP3- Teff cells out of live splenocytes. (c) FR4intCD25int-hi activated Teff cell frequency out of live splenocytes. (d) FR4loCD25lo naïve T cell frequency out of live splenocytes. (e) CD25 median fluorescence intensity (MFI) on Teffs. (f) The ratio of activated Treg cells to activated Teff cells. *significant change from control (*p < 0.05, **p < 0.001, ***p < 0.0001).
