Figure 11

CBD influences expression of synaptic scaling regulators. Y-axes = mRNA expression of BDNF, ARC/ARG3.1, and MSK1 normalized to the housekeeping control (GAPDH) and expressed as fold change from unlesioned hemispheres (2−∆∆CT). The cDNA samples synthesized from groups of n = 4 subjects were amplified in triplicate and means plotted. (a) In RA and Area X of lesioned hemispheres, CBD significantly increased mean fold expression of BDNF relative to VEH controls. (b) In HVC, RA and Area X of lesioned hemispheres CBD treatment increased mean fold expression of MSK1 over VEH. (c) In RA and Area X of lesioned hemispheres, CBD suppressed ARC/ARG3.1 expression versus VEH. Group differences were assessed by mixed-models ANOVA with Sidak’s multiple comparison correction.