Figure 1 | Scientific Reports

Figure 1

From: Genomic variability correlates with biofilm phenotypes in multidrug resistant clinical isolates of Pseudomonas aeruginosa

Figure 1

Biofilm phenotypes of different P. aeruginosa isolates. (a) Bar chart showing the result of Crystal Violet biofilm formation assay of 20 P. aeruginosa isolates. The assay was triplicated and the error bar showing the standard deviation from the mean OD for each isolate. (b) Colony morphology of SBF, MBF and WBF on Congo red (CR) containing LB agar plates (upper part) showing distinct colony morphology and Congo red release assay showing relative amount of exopolysaccharide produced by biofilms (middle part) and 120 h incubation in LB broth showing pellicle formation in air liquid interface by SBF and MBF isolates (lower part). (c) Bar chart showing the CV assay, line chart showing the attached viable cell count and CR release assay OD reading. (d) 24 h biofilms of strong (DMC-27b), moderate (DMC-20c) and weak (DMC-30b) biofilm former Pseudomonas aeruginosa. For control, 5% TSB was incubated for 24 h without bacterial inoculum. Biofilm was stained with FilmTracer live/dead stain and visualized with Olympus BX53 fluorescent microscope.

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