Table 2 qPCR cycling conditions.

From: PARP2 poly(ADP-ribosyl)ates nuclear factor erythroid 2-related factor 2 (NRF2) affecting NRF2 subcellular localization

 

Temperature

Time

Cycles

Polymerase activation

95 °C

10 min

1

Denaturation

95 °C

10 s

40

Annealing/Extension

62 °C

20 s

Melting curve analysis

95 °C

5 s

1

65 °C

1 min

65 °C to 97 °C

ramp rate 0.11 °C/s

40 °C

30 s

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