Figure 1

Schematic of the experimental setup for measuring the fluorescent response of the Fluo-4 NW dye in cells, and for tracking the luminescence of SiV centers in diamond. Two independent moving platforms are used: the coverslip seeded with living cells is fixed at the 3D-piezo stage, while the DHT is attached to the three-axis micromanipulator. The 473 nm laser beam illuminates the top side of the living cells through the low-NA objective. The excited Fluo-4 NW fluorescence collected from the bottom of the coverslip through the water-immersion objective (Nikon 60x, WI, NA = 1.27) is then either projected on an sCMOS camera or sent to the spectrometer, depending on the position of the flip mirror. The 561 nm laser was used to excite the SiV-luminescence and to induce the heating; the inset (i) shows the SEM image of a submicron glass capillary with the diamond nanoparticle localized at the tip.