Figure 1
From: Structural insights into the bi-specific cross-over dual variable antibody architecture by cryo-EM
Structure determination of the CODVIg:IL13:RefAb complex (a), Schematic representation of mono- and bispecific antibody formats. Heavy chains are in dark colors while light chains are in light colors. Constant domains (CH) are in orange, IL4 binding domains (VH2 and VL2), are in purple and IL13 binding domains (VH1 and VL1) are in green. (b), Domain organisation of CODVFab light and heavy chains. For clarity, the schematics comprises only the domains discerned in the reported structure (c), nsEM 3D reconstruction of CODVIg:IL13 obtained show no evidence of antigens bound (scale bar = 50 Å). The reconstructions are fitted with the crystal structures of CODVFab (PDB: 5HCG)11 and IgG-Fc (PDB: 4NQS)47. (d), SAXS Pair-distance distribution function (left) and scattering curve (right) for CODVIg:IL13:RefAb complex, along with a table showing several calculated values. (e), Molecular surface obtained from the aligned, averaged SAXS data fitted with atomic models of CODVFab:IL13:RefAb and IgG-Fc (PDB: 4NQS)47. The colour codes in all sub-sections of the figure are similar to that used in (a).
