Figure 2
From: Structural insights into the bi-specific cross-over dual variable antibody architecture by cryo-EM
Cryo-EM reconstruction of CODVFab:IL13:RefAb (TC) and its CODVFab:IL13 interface. (a) 3D reconstruction of TC with the fitted atomic model shown below. The colour codes of CODV domains are similar to Fig. 1a. The EM density was better-resolved for IL13 (blue) and the antigen–antibody binding interfaces and allowed for more precise modelling of the epitope-paratope residues. RefAb is in orange (heavy chain) and yellow (light chain) (b) Global resolutions estimated by the GS-FSC with a 0.143 cutoff. (c) Local resolution map of TC as estimated by ResMap. The lower resolution observed is closely related to the intra-flexibility of the Fab domains (d) Primary sequences of VH1 and VL1 domains of CODV are shown in green and that of IL13 in blue. CDR loops are underlined and the amino acids involved in hydrogen bonds are marked with asterisks. (e), Residues of CODVFab (green) and IL13 (blue) at the antigen–antibody interface within a distance of 3.5 Å.
