Figure 1
The transcription and expression feature of highly conserved gene PPP4C in vertebrates. (a) AA sequence alignment of PPP4C from human (H. sapiens), mouse (M. musculus), rat (R. norvegicus), frog (X. laevis), and zebrafish (D. rerio) species. (b) Human PPP4C transcription levels in tissues. Boxplot presents the minimum value, the first and third quartile, the median, and the maximum value in each indicated tissue. (c) Heatmap of ppp4c transcripts in X. laevis tissues. This image is generated in Xenbase (www.xenbase.org). (d) RT-PCR analysis of X. laevis ppp4c in embryos from indicated stages. n = 5. -RT without reverse transcriptase, odc1 is loading control. (e) Western blot analysis of X. laevis Ppp4c in embryos from indicated stages. n = 5. \(\upbeta\)-Actin is loading control. (f) WMISH of X. laevis ppp4c in embryos from indicated stages. n = 15. Cle. cleavage stage, Bla. blastula stage, Gas. gastrula stage, Neu. neurula stage, Tai. Tailbud stage, Sag. sagittal section, Ant. anterior, Dor. dorsal, Cro. cross section, Lat. lateral, St. Stage; 
, dorsal lip.
