Figure 7
From: Molecular basis for the recognition of 24-(S)-hydroxycholesterol by integrin αvβ3
24HC converting enzyme CYP46A1 is induced by Tumor Necrosis Factor-alpha (TNF) in macrophages, and 24HC activates a proinflammatory response in macrophages. (A) RT-PCR analyses of CYP46A1 expression in RAW 264.7 macrophages treated with TNF (100 ng/ml) for 2 h. (B) RAW 264.7 macrophages were treated with either DMSO (vehicle) or 24HC (50 µM) for 16 h. TNF production was analyzed by ELISA. The ELISA values are represented as mean ± standard deviation (n = 16 technical replicates from two independent experiments). *p ≤ 0.05 using Student’s t-test.
