Figure 8
From: Molecular basis for the recognition of 24-(S)-hydroxycholesterol by integrin αvβ3
Integrin-FAK-NFκB signaling is essential for 24HC-mediated proinflammatory response in macrophages. (A) FAK is required for 24HC-mediated proinflammatory response. RAW 264.7 macrophages were treated with 24HC (50 µM) for 16 h in the presence of either DMSO (vehicle control) or FAK inhibitor PF-431396 (5 mM). TNF secretion was analyzed by ELISA. (B) NFκB is required for 24HC-mediated proinflammatory response. RAW 264.7 macrophages were treated with 24HC (100 µM) for 16 h in the presence of either DMSO (vehicle control) or NFκB inhibitor BAY-11 (1 μM). TNF secretion was analyzed by ELISA. The ELISA values are represented as mean ± standard deviation (n = 14–24 technical replicates from two–three independent experiments). *p ≤ 0.05 using Student’s t-test.
