Figure 1
Comparison between static versus perfusion culture: (A) Schematic representation of static culture of RH30 cells seeded on Ultrafoam (collagen I sponge). (B) H&E staining of Ultrafoam with RH30 cells obtained after 4, 7 and 15 days of static culture. Scale bar 1 mm. (C) Cell distribution in Ultrafoam in static condition. The image of the whole scaffold has been divided in 5 sections along the direction of the perfusion. Sections were enumerated from 1 (starting from the top of the scaffold) to 5 (including the bottom of the scaffold). N = 3 scaffolds for each condition have been analyzed. (D) Schematic representation of the bioreactor. Perfusion culture (dynamic condition) of RH30 cells seeded on Ultrafoam. (E) H&E staining of Ultrafoam with RH30 cells obtained after 4, 7, and 15 days of perfusion culture. Scale bar upper row: 1 mm; lower row: 100 μm. (F) Cell distribution in Ultrafoam in perfusion condition.
