Figure 4

Study, in static and perfusion conditions, of the proteins linked to tumor invasion and metastasis. (A) MMP-2 gene expression on static and perfusion samples cultured for 7 days. *P < 0.05. (B) left: Zymography on static and perfusion samples cultured for 7 days. Right: quantification of MMP-2 in zymography gel. **P < 0.01. (C) Total protein levels in static, perfusion, and xenograft are represented in the histograms. RPPA analysis of the upper branch of the pathway involving ITGB1 and FAK and of the lower branch of the pathway involving CDC42, n-WASP, and ARP2. (N = 5). *P < 0.05; **P < 0.01; ***P < 0.001.