Figure 5

Reduced P-selectin exposure upon activation and increased number of α-granules in Strumpellin-deficient platelets. (a) Transmission electron micrographs from resting control and Strumpellin-deficient platelets. Exemplary white arrows point to α-granules. Scale bar indicates 3 µm. (b) Number of α-granules per platelet based on evaluation of transmission electron micrographs as shown in (a). Values are mean ± s.d.; in total at least 121 platelets from three control and three Strumpellin-deficient mice were analyzed (n = 3; **P < 0.01). The experiments in (a–c) were performed once. (c) Area of α-granules in platelets of control and Strumpellin-deficient mice based on transmission electron micrographs (a). Values are mean ± s.d. of at least 121 platelets (n = 3). (d) Determination of P-selectin exposure on the surface of resting platelets and upon stimulation with adenosine diphosphate (ADP), U46619 (U46, thromboxane analogue), thrombin (Thr), collagen related peptide (CRP), convulxin (CVX) and rhodocytin (Rhod). Values are mean ± s.d. (n = 6; *P < 0.05; **P < 0.01). (e) P-selectin content of resting control and Strumpellin-deficient platelets. Values are mean ± s.d. (n = 6). Dashed red line indicates the mean value from P-selectin-deficient platelets.