Figure 5
From: Regulation of TRIB1 abundance in hepatocyte models in response to proteasome inhibition
In the presence of BTZ, loss of polyUb in response to CHX is slower compared to TRIB1. Western blot analysis of HuH7-T1 and HepG2-T1 treated with the indicated drugs for 5 h. (A) Representative Western blots. Levels of polyUb were quantified over the 100–150 kDa range. (B) Relative sensitivities of TRIB1 and polyUb signals to BTZ and CHX after a 5 h treatment. Quantifications of the TRIB1 signals and polyUb (TUBB corrected) in HepG2-T1 and HuH-7-T1 cells following BTZ and/or CHX treatments. Dashed line indicates a no-loss scenario. Average of 3 independent experiments. *p < 0.05; **p < 0.01; Unpaired T test, TRIB1 versus polyUb.
