Figure 6
From: Regulation of TRIB1 abundance in hepatocyte models in response to proteasome inhibition
Pulse chase of recombinant TRIB1 in HepG2 cells. Left, representative Western blot of HepG2-T1 cells treated with 5 µM BTZ or vehicle (0.5% DMSO) for 2 h, followed by a wash out period of 0 to 5 h in the presence of CHX (10 µg/ml) or no CHX (recovery). Right, best fit regressions of biological triplicates using the one phase decay model (GraphPad Prism). TRIB1 abundance (TRIB1/TUBB) values were normalized to the matching control value (Vehicle or BTZ). Error bars for the [BTZ, 2 h] time point are contained within the symbol.
