Figure 3
From: RNA activation in ticks
Confirmation of HlemCHT activation by dsRNA targeting the 3ʹ-UTR. (a) Relative gene expression of HlemCHT at 13 days post-oviposition (dpo). Data is represented by a histogram. Relative expression of the H. longicornis 40S ribosomal gene was used to normalize the HlemCHT gene expression data. Error bars represent the SEM of 4 replicates. *P = 0.0145. (b) Representative stereomicroscopic images of dsRNA-derived eggs at 13 dpo. (c) Representative stereomicroscopic images of dsRNA-derived eggs hatching at 20 and 21 dpo. (d) Histogram showing the comparison of the hatching rate of dsHlemCHT-derived eggs, as against eggs of dsEc-malE at 21 dpo. Error bars represent the SEM of 4 replicates. The results shown are from a single experiment and are representative of three independent experiments.
