Table 1 List of primers.
From: RNA activation in ticks
Primers | Position | Primer sequence | Tm ( °C) |
|---|---|---|---|
HlemCHT 5'-complete ORF | Forward | TGTATGCGTTCAGTGCTACCGGGCGTA | 65.1 |
Reverse | TAAAGCCAGCCCAGTTCCCACGCAGAT | 65.1 | |
HlemCHT 5 '-TOPO colony PCR | Forward | TGGTTGGGCTGAGGGCGGAAAGAAGTA | 65.1 |
Reverse | TGTAGACGAAACTTGGCGACGGGCACA | 65.1 | |
HlemCHT 5'-complete ORF sequencing | Forward | TGGTTGGGCTGAGGGCGGAAAGAAGTA | 65.1 |
Reverse | TGTAGACGAAACTTGGCGACGGGCACA | 65.1 | |
Full HlemCHT ORF | Forward | ACGAGCTGTGCGTGCTTGCCAAAGTCT | 65.1 |
Reverse | TGCAGGCTAGTTTTTCCGGCTAGCCACTC | 66.2 | |
Full HlemCHT ORF sequencing | Forward | CGGGGAGGTGCATACTCTGACAGGGCTAA | 67.6 |
Reverse | CGGTGTTGCCCTTGTCACTCAGCGTGT | 66.6 | |
HlemCHT (3ʹ-UTR) dsRNA synthesis (T7) | Forward | *gaaattaatacgactcactataggTCACATGCTCACGATCACCCGCATACC | 65.1 |
Reverse | *gaaattaatacgactcactataggGGACGTGTCCTTGCACGTTGGCATACA | 65.1 | |
HlemCHT (3ʹ-UTR) dsRNA synthesis (Gene-specific) | Forward | TCACATGCTCACGATCACCCGCATACC | 65.1 |
Reverse | GGACGTGTCCTTGCACGTTGGCATACA | 65.1 | |
HlemCHT (ORF) dsRNA synthesis (T7) | Forward | *gaaattaatacgactcactataggCAAGGGCAACACCGGACTCAGGGCTTA | 66.6 |
Reverse | *gaaattaatacgactcactataggGAACATCACCATGGGCGTCCCGTACAC | 66.6 | |
HlemCHT (ORF) dsRNA synthesis (Gene-specific) | Forward | CAAGGGCAACACCGGACTCAGGGCTTA | 66.6 |
Reverse | GAACATCACCATGGGCGTCCCGTACAC | 66.6 | |
MalE gene | Forward | CGACCGCTTTGGTGGCTACGCTCAATC | 66.6 |
Reverse | CCGCAGTACGCACGGCATACCAGAAAG | 66.6 | |
MalE dsRNA synthesis (T7) | Forward | *gaaattaatacgactcactataggGTTGGCTGAAATCACCCCGGACAAAGC | 65.1 |
Reverse | *gaaattaatacgactcactataggTTAATACCTGCGCTCAGCACGCCAACG | 65.1 | |
MalE dsRNA synthesis (Gene-specific) | Forward | GTTGGCTGAAATCACCCCGGACAAAGC | 65.1 |
Reverse | TTAATACCTGCGCTCAGCACGCCAACG | 65.1 | |
Full HlemCHT ORF-TOPO colony PCR / HlemCHT RT-qPCR | Forward | CATACCGCACGAGAACGACTGCACCAA | 65.1 |
Reverse | CACAGTTGCCGTTGTCCTGGTTCCACA | 65.1 | |
40S ribosomal S3a RT-qPCR | Forward | CCTCATCCGCAAGGTGAAGGTGCTCAA | 65.1 |
Reverse | AAGCGCCCTCAGACATTCTCCAGCACA | 65.1 |
