Table 1 Primer sequence for RT-PCR, Complex I: nicotinamide adenine dinucleotide dehydrogenase (ND1 and ND2); Complex III: cytochrome b (CYTb); Complex IV: cytochrome c oxidase (CO1); Complex V: ATPase 6 and 8 subunits (ATPase 6).
From: Impact of adrenomedullin on mitochondrial respiratory capacity in human adipocyte
Forward | Reverse | |
|---|---|---|
ND1 | 5’-GGGCTACTACAACCCTTCGCT-3’ | 5’-GAGGCCTAGGTTGAGGTTGAC-3’ |
ND2 | 5’-CACAGAAGCTGCCATCAAGTA-3’ | 5’-CCGGAGAGTATATTGTTGAAGAG-3’ |
CYTb | 5’-TCATCGACCTCCCCACCCCATC-3’ | 5’-CGTCTCGAGTGATGTGGGCGATT-3’ |
CO1 | 5’-TCATGATCACGCCCTCATA-3’ | 5’-CATCGGGGTAGTCCGAGTAA-3’ |
ATPase 6 | 5’-GCCCTAGCCCACTTCTTACC-3’ | 5’-TTAAGGCGACAGCGATTTCT-3’ |
18S | 5’-TTCGAACGTCTGCCCTATCAA-3’ | 5’-ATGGTAGGCACGGCGACTA-3’ |
GAPDH | 5’-GGTCTCCTCTGACTTCAACA-3’ | 5’-AGCCAAATTCGTTGTCATAC-3’ |
