Figure 4
From: Sex-specific effects of Cre expression in Syn1Cre mice
Cre transgene triggers increased hGH expression leading to a sex-specific reduction in body size of male Syn1Cre mice. (a) Two-way ANOVA analysis of Syn1Cre body weight revealed a sex-specific significant reduction in Syn1Cre males. (b) Two-way ANOVA analysis of femur length revealed a sex-specific reduction in male Syn1Cre femur size. (c) Measurement of hGH transcript levels using quantitative real-time PCR (qPCR) in the hypothalamus revealed significant increase in hGH expression in Syn1Cre mice. (d) No significant difference in Ghrh levels in hypothalamus extracts between Syn1Cre mice and wild type littermates. (e) Significant increase in Cish transcript levels in the hypothalamus of Syn1Cre mice. (g) Measurement of CD36 transcripts by qPCR in liver tissue revealed no significant difference between Syn1Cre mice and wild type littermates. (f) Two-way ANOVA of Igf1 transcript levels in Syn1Cre mice revealed a sex-specific reduction of hepatic Igf1 expression in male Syn1Cre mice. Number of animals reported at the bottom of the bars. All error bars correspond to standard deviation of the mean. Asterisks denote the following: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. For detailed statistical values see Supplementary Table S1.
