Figure 5

Syn1Cre expression has no effect on glucose homeostasis or energy expenditure. Glucose tolerance test (GTT) on male Syn1Cre mice (a) and female Syn1Cre mice (b) did not detect any significant difference relative to their respective wild type littermates. Insulin sensitivity test (ITT) on male Syn1Cre mice (c) and female Syn1Cre mice (d) did not detect any significant difference relative to their wild type littermates. ANCOVA on day-time energy expenditure of Syn1Cre mice showed no significant difference between male (e) or female (f) Syn1Cre and their respective wild type littermates (male wild type and Syn1Cre n = 8, female wild type n = 7 and Syn1Cre n = 9). Locomotor activity of single-housed male (g) and female (h) Syn1Cre mice plotted against zeitgeber revealed no significant difference between Syn1Cre mice and wild type littermates. Number of animals reported at the bottom of the bars. All error bars correspond to standard deviation of the mean. For ANCOVA analysis the 95% confidence interval is plotted. Asterisks denote the following: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. For detailed statistical values see Supplementary Table S1.