Figure 5 | Scientific Reports

Figure 5

From: Glutamine synthetase (GS) knockout (KO) using CRISPR/Cpf1 diversely enhances selection efficiency of CHO cells expressing therapeutic antibodies

Figure 5

A workflow of the stable cell line generation process in this study. The figure illustrates major steps in clonal screening procedure and stability assessments of top clones. The mAb producing rCHO clones were created from different GS-KO CHO host cell lines generated in this study. A single round of selection with 25 µM MSX in Gln-free medium was conducted. Clones selected from different host cell lines were analyzed with respect to Qp and long-term production stability over more than 60 generations.

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