Figure 2 | Scientific Reports

Figure 2

From: Reduction in disialyl-T antigen levels in mice deficient for both St6galnac3 and St6galnac4 results in blood filling of lymph nodes

Figure 2

Phenotypes of abnormal lymph nodes in DKO mice. (A) Frequency of blood-filled lymph nodes in KO mice. LN: Lymph node. WT: n = 33, 3KO: n = 38, 4KO: n = 19, DKO: n = 28. (B) HE staining of the submandibular lymph nodes in DKO mice. Representative pictures of the subcapsular sinus, medullary sinus, and paracortex in LNs. (C) Immunostaining results of mesenteric lymph nodes in 3KO (littermate control) and DKO mice. Red arrow: red blood cells within the HEV area. Blue arrow: red blood cells flowed in the lymphatic vessel. Podoplanin (green): a marker for lymphatic endothelial vessels and fibroblastic reticular cells. Ter119 (red): a marker for red blood cells. Lyve-1 (blue): a marker for the lymphatic endothelial vessels. (D) Comparison of mesenteric lymph nodes and lymphatic vessels between 3KO and DKO mice. High magnification images (lower panels) of mesenteric lymphatic vessels in 3KO and DKO mice. Lower panels show a magnified image of the inset marked in the upper panels. Arrows indicate the range of mesenteric lymph nodes. (E) Comparison of the thoracic duct (white arrows) between 3KO and DKO mice. Lower panels are magnified images of the area marked in the upper panels.

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