Figure 5 | Scientific Reports

Figure 5

From: Molecular cloning, prokaryotic expression and its application potential evaluation of interferon (IFN)-ω of forest musk deer

Figure 5

Expression, purification and confirmation of rIFNω proteins from E. coli BL21. (A) SDS-PAGE results of preliminary induction of rIFNω expression. Note: M, protein molecular weight standard (15–120 kDa); 1, uninduced bacteria with untreated pGEX-6P-1; 2, induced bacteria with untreated pGEX-6P-1; 3, uninduced rBL21; 4, induced rBL21; 5, the supernatant of induced rBL21 after ultrasonic crushing; 6, precipitation of induced rBL21 after ultrasonic crushing. (B) Purification result of rIFNω. Note: M, protein molecular weight standard (11–180 kDa); 1–3, flow through liquid; 4–5, rinse liquid; 6–8, eluent; 9, ultrafiltration concentrate rIFNω; 10, filtrate. (C) Western blot confirmation. Note: 1, purified rIFNω; 2, induced bacteria with untreated pGEX-6P-1. M, Protein molecular weight standard (15–120 kDa).

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