Figure 1

A low-protein diet improves glucose homeostasis in lipodystrophic diabetic mice. Blood glucose (A, B) and body weight (C, D) values of IR^FKO (circles) and WT (triangles) mice fed ad libitum low protein diet (solid lines) or chow diet as control (empty lines) in males (blue) and females (red). Measurements were performed at fed conditions every 3 days for 2 weeks of diet treatments. (E) Intraperitoneal glucose tolerance test for WT and IRKFO male mice fed for 2 weeks with LPD or chow diet. In short, blood glucose was measured before (time = 0) and 15, 30, 60, 90, 120 min after intraperitoneal injection bolus of 2 g/kg dextrose. Values are expressed in mg/dL. (F) Intraperitoneal insulin tolerance test for male IR^FKO and WT mice injected intraperitoneally with 1.5U/kg insulin. Blood glucose was measured before injection (time = 0) and at 15, 30, 60, 90, 120 min. Values are expressed as % of initial blood glucose. (G) Insulin, (H) triglycerides and (J) non-sterified fatty acids serum values of IR^FKO and WT male mice. N = 5–8 mice for each condition in all experiments displayed. All data are presented as the mean ± standard error of mean (SEM) and were analyzed by two-way ANOVA test with a threshold for significance of 0.05 adjusted P value. * = P > 0.05; ** = P > 0.01; *** = P > 0.001;**** = P > 0.0001.