Figure 2

LPD activates BAT glucose uptake and thermogenesis. Absolute (A) and relative (B) tissue weights for male IR^FKO and WT mice fed for 2 weeks with chow diet (empty lines) or LPD (solid lines). (C) Representative images of H&E-stained sections of liver from male IR^FKO and WT mice fed for 2 weeks with chow diet or LPD. Scale bar, 50 µm. (D) Liver triglycerides (TG) and non-esterified fatty acid (NEFA) content for male IR^FKO and WT mice fed for 2 weeks with chow diet (empty lines) or LPD (solid lines). (E) Representative images of H&E-stained sections of BAT from male IR^FKO and WT mice fed for 3, 7 and 14 days with chow diet or LPD. Scale bar, 100 um. (F) BAT 2-DG glucose uptake for male IR^FKO and WT mice fed for 2 weeks with chow diet (empty lines) or LPD (solid lines). Values are expressed as DPM per mg of tissue. (G) Ucp1 mRNA levels in BAT of male IR^FKO and WT mice fed for 2 weeks with chow diet (empty lines) or LPD (solid lines). Values are normalized to TBP gene levels. N = 5–8 per group for (A–E, G). N = 3 per group for F. All data are presented as the mean ± standard error of mean (SEM) and were analyzed by two-way ANOVA test with a threshold for significance of 0.05 adjusted P value except in (F) were comparison between IR^FKO chow versus IR^FKO LPD was performed on separate two-tail student t test. *P > 0.05; **P > 0.01; ***P > 0.001; ****P > 0.0001; ^###P > 0.001.