Table 1 Summary of dog experiments. Each dog (Golden Retriever, GR; Golden Retriever Muscular Dystrophy, GRMD; MuStem cell-treated GRMD, GRMDT) was assigned an identification number. The age of dogs at sacrifice, initiation of immunosuppression and 3 cell injections is expressed in weeks. Cause of death, the nature and status (none or continuous) of immunosuppressant treatment, number of cells injected, and duration of transplantation protocol are indicated. Details of the experiments carried out in each of the 12 dogs are provided. CsA, Cyclosporin A (Neoral®; Novartis); MMF, mycophenolate mofetil (CellCept®; Roche); IF, immunofluorescence; DUV, deep ultraviolet; wDUV, widefield DUV; sDUV, spectroscopy DUV. “X” indicates the experiments that have been carried out. “*” indicates that a score of 100% is obtained by all healthy GR dogs.
Animals | Immunosuppression regimen | Cell transplantation | Clinical follow-up | Tissue analysis | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Group | ID number | Age at sacrifice | Cause of death | Drugs used | Age at initiation | Cell number (107/kg) | Age at different injections | Scoring | Histology staining | Deep ultraviolet radiation Synchrotron | ||
IF | wDUV | sDUV | ||||||||||
GRMDT | 1 | 40.0 | End of protocol | CsA/MMF | 16.6 | 7.4; 8.0; 7.1 | 17.6; 20.1; 22.0 | X | X | X | X | X |
2 | 40.1 | End of protocol | CsA/MMF | 18.0 | 7.8; 5.5; 7.2 | 19.0; 20.7; 22.4 | X | X | X | X | X | |
3 | 39.7 | End of protocol | CsA/MMF | 11.1 | 8.0; 6.7; 7.7 | 12.1; 13.6; 15.1 | X | X | X | X | X | |
4 | 37.9 | End of protocol | CsA/MMF | 15.1 | 6.7; 5.8; 6.9 | 16.1; 17.7; 19.1 | X | X | X | X | ||
GRMD | 5 | 47.6 | End of protocol | CsA/MMF | 18.7 | None | – | X | X | X | X | X |
6 | 37.9 | End of protocol | CsA/MMF | 10.7 | None | – | X | X | X | X | X | |
7 | 33.6 | Ethical reason | CsA/MMF | 10.6 | None | – | X | X | X | X | X | |
8 | 47.4 | End of protocol | CsA/MMF | 17.6 | None | – | X | X | X | X | ||
GR | 9 | 41.5 | End of protocol | None | – | – | – | None; * | X | X | X | X |
10 | 41.5 | End of protocol | None | – | – | – | None; * | X | X | X | X | |
11 | 36.0 | End of protocol | None | – | – | – | None; * | X | X | X | X | |
12 | 36.1 | End of protocol | None | – | – | – | None; * | X | X | X |