Table 1 Summary of dog experiments. Each dog (Golden Retriever, GR; Golden Retriever Muscular Dystrophy, GRMD; MuStem cell-treated GRMD, GRMDT) was assigned an identification number. The age of dogs at sacrifice, initiation of immunosuppression and 3 cell injections is expressed in weeks. Cause of death, the nature and status (none or continuous) of immunosuppressant treatment, number of cells injected, and duration of transplantation protocol are indicated. Details of the experiments carried out in each of the 12 dogs are provided. CsA, Cyclosporin A (Neoral®; Novartis); MMF, mycophenolate mofetil (CellCept®; Roche); IF, immunofluorescence; DUV, deep ultraviolet; wDUV, widefield DUV; sDUV, spectroscopy DUV. “X” indicates the experiments that have been carried out. “*” indicates that a score of 100% is obtained by all healthy GR dogs.

From: Specific and label-free endogenous signature of dystrophic muscle by Synchrotron deep ultraviolet radiation

Animals

Immunosuppression regimen

Cell transplantation

Clinical follow-up

Tissue analysis

Group

ID number

Age at sacrifice

Cause of death

Drugs used

Age at initiation

Cell number (107/kg)

Age at different injections

Scoring

Histology staining

Deep ultraviolet radiation Synchrotron

IF

wDUV

sDUV

GRMDT

1

40.0

End of protocol

CsA/MMF

16.6

7.4; 8.0; 7.1

17.6; 20.1; 22.0

X

X

X

X

X

2

40.1

End of protocol

CsA/MMF

18.0

7.8; 5.5; 7.2

19.0; 20.7; 22.4

X

X

X

X

X

3

39.7

End of protocol

CsA/MMF

11.1

8.0; 6.7; 7.7

12.1; 13.6; 15.1

X

X

X

X

X

4

37.9

End of protocol

CsA/MMF

15.1

6.7; 5.8; 6.9

16.1; 17.7; 19.1

X

X

X

X

 

GRMD

5

47.6

End of protocol

CsA/MMF

18.7

None

X

X

X

X

X

6

37.9

End of protocol

CsA/MMF

10.7

None

X

X

X

X

X

7

33.6

Ethical reason

CsA/MMF

10.6

None

X

X

X

X

X

8

47.4

End of protocol

CsA/MMF

17.6

None

X

X

X

X

 

GR

9

41.5

End of protocol

None

None; *

X

X

X

X

10

41.5

End of protocol

None

None; *

X

X

X

X

11

36.0

End of protocol

None

None; *

X

X

X

X

12

36.1

End of protocol

None

None; *

X

X

X